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  4. Biotechnology and its Applications Chapter-Wise Test 2

Biotechnology and its Applications Chapter-Wise Test 2

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which technique is based on antigen-antibody interaction for disease diagnosis?

PCR
ELISA
Northern blotting
DNA fingerprinting
2

ELISA detects the presence of antigens or antibodies in a sample through antigen-antibody interaction.

Which method is used to amplify a specific DNA segment?

Southern blotting
Polymerase Chain Reaction (PCR)
Micropropagation
Autoradiography
2

Polymerase Chain Reaction (PCR) is used to amplify specific DNA segments in vitro.

Which molecule is used as a probe in Southern blotting for DNA detection?

mRNA
DNA probe
tRNA
Protein
2

DNA probes are used in Southern blotting to detect specific DNA sequences through hybridization.

Which of the following statements about tissue culture is incorrect?

Tissue culture allows mass production of plants.
Tissue culture requires sterile conditions.
Tissue culture produces genetically different plants.
Tissue culture can regenerate whole plants from a single cell.
3

Tissue culture produces genetically identical plants (clones), not genetically different plants.

Which vector is derived from Agrobacterium tumefaciens and is commonly used in plant genetic engineering?

Plasmid pBR322
Ti plasmid
Lambda phage
Retrovirus
2

Ti plasmid derived from Agrobacterium tumefaciens is widely used as a vector in plant genetic engineering.

Which term refers to genetically identical individuals produced through tissue culture?

Hybrids
Somaclones
Transgenics
Mutants
2

Somaclones are genetically identical individuals produced through tissue culture techniques.

Which of the following is correct about Bt toxin in pest-resistant crops?

Bt toxin is harmful to all living organisms.
Bt toxin is produced by Bacillus thuringiensis.
Bt toxin is produced by plants naturally without modification.
Bt toxin prevents viral infections in crops.
2

Bacillus thuringiensis produces Bt toxin, which is introduced into crops through genetic engineering for pest resistance.

Which of the following is incorrect about cry genes in Bt crops?

Cry genes are derived from Bacillus thuringiensis.
Cry genes produce proteins that kill insect larvae.
Cry genes improve the nutritional content of crops.
Cry genes are introduced into crops for pest resistance.
3

Cry genes provide pest resistance by producing insecticidal proteins; they do not improve the nutritional content of crops.

Which plant was modified using RNA interference to develop resistance against Meloidogyne incognita?

Tomato
Tobacco
Rice
Potato
2

Tobacco plants were modified using RNA interference to develop resistance against the nematode Meloidogyne incognita.

Which of the following is correct regarding DNA probes in molecular diagnostics?

DNA probes are used to cut DNA at specific sites.
DNA probes are used to detect specific DNA sequences.
DNA probes synthesize proteins in cells.
DNA probes inhibit RNA synthesis in bacteria.
2

DNA probes are used in molecular diagnostics to detect specific DNA sequences by hybridizing with complementary sequences.

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